Journal: Oncology Letters
Article Title: SMOC1 silencing suppresses the angiotensin II-induced myocardial fibrosis of mouse myocardial fibroblasts via affecting the BMP2/Smad pathway
doi: 10.3892/ol.2018.8989
Figure Lengend Snippet: SMOC1 silencing downregulates the expression levels of fibrosis-associated proteins. (A) ELISA was performed to measure the TGF-β1, COL-I and COL-III expression in MFBs, MFBs transfected with an empty vector, and Ang II-induced MFBs transfected with an empty vector or si-SMOC1. (B) Reverse transcription-quantitative polymerase chain reaction and (C) western blot analysis were performed on the expression levels of FN, TGF-β1, COL-I and COL-III in MFBs, MFBs transfected with an empty vector, and Ang II-induced MFBs transfected with an empty vector or si-SMOC1. *P<0.05, **P<0.01, and ***P<0.001 vs. NC; ^ P<0.05 and ^^ P<0.01 vs. Ang II+NC. SMOC1, SPARC-related modular calcium binding 1; FN, fibronectin; TGF-β1, transforming growth factor β1; COL, collagen; Ang II, angiotensin II; MFBs, myocardial fibroblasts; si, small interfering RNA; NC, negative control.
Article Snippet: The expression of TGF-β1, COL-I and COL-III were determined by ELISA kits: Mouse TGF-β1 ELISA Kit (E-EL-M0051c; Elabscience, Wuhan, Hubei, China), COL1 ELISA kit (E-EL-M0325c; Elabscience) and COL-III ELISA kit (E-EL-M0316; Elabscience).
Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Transfection, Plasmid Preparation, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Binding Assay, Small Interfering RNA, Negative Control